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Fig. 3. Altered expression of Nkx2.2 in Nkx6.1 mutant spinal cord at the thoracic level. (A-F) Spinal cord sections from E13.5 (A,B), E15.5 (C,D) and E18.5 (E,F), wild-type (A,C,E) and mutant (B,D,F) animals were hybridized with Nkx2.2 riboprobe. (G-L) Double immunofluorescent staining of E13.5 (G,H) and E18.5 (I-L) spinal cord sections with anti-Nkx2.2 and anti-Olig2 (G-J), or with anti-Nkx2.2 and NeuN (K,L). In the ventral gray matter, most Nkx2.2+ cells co-express NeuN but not Olig2. In the white matter, Nkx2.2+ cells co-express Olig2, but not NeuN. Representative Nkx2.2+ cells are indicated by arrowheads, whereas the double-positive cells are indicated by arrows. (M,N) Higher power view of the NeuN/Nkx2.2 double-positive cells (yellow) in the wild-type (M) and Nkx6.1 mutant (N) spinal cords.





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