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Fig. 4. (A-F) Disrupted expression of Mbp and Plp in Nkx6.1 mutant spinal cord. Spinal cord sections at the thoracic level were prepared from E17.5 (A,B) and P0 (C-F) wild-type (A,C,E) and mutant (B,D,F) animals and hybridized with Mbp (A-D) and Plp (E,F) riboprobes. (G-H) Similar MBP expression in spinal cord explants in normal and Nkx6.1 mutants. Spinal cord explants were isolated from the thoracic regions of the E13.5 wild-type (G) and mutant (H) embryos and cultured in vitro for 7 days before whole-mount in situ hybridization with Mbp riboprobe.





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