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Fig. 2. Gli2–/–Gli3–/–
mutant mice exhibit a severe loss of sclerotomal gene expression. Expression
of Pax1, Pax9 and Sox9 was assessed by whole-mount in situ
hybridization in wild type (A,A',A''),
Gli2–/– (B,B',B''),
Gli3–/– (C,C',C''),
Gli2–/–Gli3+/–
(D,D',D'') and
Gli2–/–Gli3–/–
(E,E',E'') E9.5 embryos. Pax1 and Pax9 are
expressed normally in all allelic combinations (A-D,A'-D') except
for
Gli2–/–Gli3–/–
embryos, which exhibit a severe reduction and delay in Pax1 and
Pax9 (between arrowheads in E and E') expression. In
Gli3–/– embryos Sox9 expression is
reduced in the anterior somites, with the dorsal region most affected (compare
arrowheads in inset of A'' and C'').
Gli2–/– and
Gli2–/–Gli3+/–
embryos exhibit reduced Sox9 in both ventral and dorsal domains of
anterior somites (arrowheads in B'' and D''). Sox9 appears
normal in the posterior somites of these embryos (indicated by the black line
A''-D''). Sox9 is undetectable in
Gli2–/–Gli3–/–
embryos. Scale bar: 0.5 mm.
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