|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. RNAi of dally-like and dally do not inhibit Wingless
signalling. (A-C) Late stage 11 embryos. en transcription revealed by
in situ hybridisation in wild type (A) and in embryos ectopically expressing
wg (B,C). In armGal4/UASwg embryos, the ubiquitous activation of
wg signalling stimulates en transcription in a competence
domain spanning half a segment (B). The ectopic transcription of en
is unaffected in embryos of the same genotype injected with dlp dsRNA
(n=83), showing that dlp is not required for Wg signalling
(C). (D-F) Cuticle preparations of simGal4/UASwg embryos, where the cells of
the ventral midline express wg. (D) In response to Wg signalling, the
cells in the ventral-most portion of the denticle belts secrete a naked
cuticle. (E) In embryos of the same genotype injected with dlp dsRNA,
a segment polarity phenotype appears in the ventral-lateral portions of the
abdomen (87%, n=119), showing that dlp has been silenced
efficiently. However, naked cuticle is still produced at the midline,
indicating that ectopic Wg signalling is unaffected by the loss of
dlp. (F) The same proportion of segment polarity phenotypes is found
when dlp and dally dsRNA are injected together in
simGal4/UASwg embryos (81%, n=67). As occurs with dlp single
injection, naked cuticle secretion is unaffected at the midline, showing that
these genes are not required in a redundant manner for Wg signalling.
|
