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Fig. 4. shark follicle-cell mosaics produce two distinct DA phenotypes.
(A,B) DIC images of shark1-mosaic egg chambers. (A) DA
chorion defect. (B) Short DA with normal chorion. (C-F) DIC and confocal
images of a single egg chamber in which only the shark1
clonal cells are GFP positive. GFP-positive cells are green and
anti-
-Spectrin in red shows cell membranes. Asterisk in C indicates
abnormal `thumb' of chorion in anterior of the right DA (C,D). The left DA
(E,F) is vacuolated. (G) Diagrams of six confocal z slices taken 3
µm apart of egg chamber in C-F. Arrows indicate the corresponding
z slice for the two confocal images shown. GFP-positive cells are
labeled green. A region where the cell boundary is indeterminate is shown in
purple. shark1 clonal cells lie between the two DA arms or
are closely associated with the DA shown in E,F. (H) Table showing frequency
of clones and defects.
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