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Fig. 1. Expression of genes that are deregulated in ß-catenin-/-
embryos, as determined by microarray analysis. (A) Transcriptional changes
that occur between E6.0 and E6.5 in wild-type embryos, and comparison with
ß-catenin mutants. The expression of 130 genes is downregulated, and
expression of 673 genes is upregulated in wild-type embryos between these two
stages. In ß-catenin-/- embryos, most genes are similarly
modulated (green; 127 are also downregulated and 591 are also upregulated). A
minority of genes are oppositely regulated (red; three genes are downregulated
and 82 genes upregulated). (B) Upper panel: dissection scheme for separation
of embryonic tissues. X, extra-embryonic tissues (green); VE, visceral
endoderm (yellow); EE, embryonic ectoderm including primitive streak region
(blue); M, mesoderm (red). Lower panel: assessment of the expression of genes
that are known to be expressed in a tissue-specific manner. Pou5f1 is
expressed in EE, brachyury (T) in EE and M, Cer1 in VE, and
Bmp4 in X (Wilkinson et al.,
1990; Rosner et al.,
1990; Belo et al.,
1997; Lawson et al.,
1999). Red, high relative expression; blue, low relative
expression; bright area, genes with consistent measurements; dark area, genes
with variable or low measurements in certain tissues. (C) Assessment of the
tissue specificity of expression of genes that are deregulated in
ß-catenin-/- embryos, as determined by expression profiling.
Genes that are deregulated at E6.0 or E6.5 greater than twofold (Change
p-Value<0.05) are displayed. Names of selected genes are indicated on the
right.
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