|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. ed mutations promote generation of extra bristles in the notum of
Drosophila. (A-C) Nota from wild-type,
ed1X5/edslH8 and sca-Gal4;
UAS-edIII flies, respectively. On B, note the extra
macrochaetae arising near the extant ones and the increased density of
microchaetae with respect to A. (C) Expression of
UAS-edIII partially suppressed macrochaetae (such as the
dorsocentrals and scutellar, and some microchaetae) and also caused occasional
duplication of some macrochaetae (arrowhead). (D,E,E') Prospective notum
of a third instar wing disc harbouring ed1x5 homozygous
clones (absence of the GFP green fluorescence, arrowheads). Boxed area in A is
shown at greater magnification in E and E' (E', green channel
only). Note the very small size of the clones, which in some cases consist of
a single cell (arrowheads in D,E,E') that has been singled out as an SOP
(red, Sens marker). One of the two ed1x5 SOPs corresponds
to an ectopic SO, because only two SOPs arise from a wild-type DC proneural
cluster. The twin wild-type clones (bright green) consist of many cells,
indicating the poor viabilty of the ed1x5 homozygous
cells. (F) An extra DC f36a ed1x5 macrochaeta
(red arrowhead) that may have arisen from a clone similar to those in E, but
which probably consisted of more than a single cell, as two mutant
microchaetae have developed adjacent to the extra macrochaeta. Black
arrowheads indicate the ADC and PDC macrochaetae. (G) ed1x5
M+ clones, induced at 72-96 hours after egg laying (absence of
green marker) survive well in a M+/– background and
promote development of extra SOPs (red, Sens marker) when they include cells
of a proneural cluster, in this case the DC one. No extra SOPs were observed
outside the ed1x5 M+ clones. (H,I) Groups of
extra bristles (labeled with f36a) develop within
ed1x5 M+ clones. Five f36a
DC macrochaetae are shown in H (red arrow) and a patch of
f36a microchaetae in I (red arrow; clones were induced at
48-72 hours after egg laying). The increased density of microchaetae can be
seen by comparing with the same region of a wild-type notum (J). Asterisks
indicate the ADC macrochaeta. Black arrowhead in H indicates a
f+ DC macrochaeta displaced from its position by the
ed1x5 extra macrochaetae. (K,L) Prospective wing of third
instar discs harbouring ed1x5 homozygous clones (absence
of the GFP green fluorescence) in a wild-type or
UAS-edIII/C765-Gal4 background, respectively. The
expression of UAS-edIII largely increased the number of
surviving clones and that changed their contours from smooth to uneven.
|
