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Fig. 1. Deletion of the Fgf3-coding region in mice. (A) The genomic locus
with the exons and coding regions of the Fgf3 gene indicated. The
coding region was replaced by a FGF3 cDNA and a neor gene
flanked by loxP sites by homologous recombination. The introduced sequences
were removed by Cre-mediated excision between the external loxP sites. (B)
Southern blot analysis was carried out using the probes indicated in A to
detect the correct targeting event in ES cells and subsequent deletion of the
introduced sequences. (C) PCR analysis using primer pairs indicated by pink
arrows in A demonstrating the presence and absence of specific products
amplified from the targeted locus and after generation of the knock out
allele. (D) Whole-mount RNA hybridisation analysis of Fgf3 expression
in the hindbrain of an E8.5 Fgf3–/– mutant
embryo and a wild-type littermate. Scale bar: 100 µm.
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