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Fig. 1. Cyclin A protein expression in the germarium. (A, left) The four mitotic
divisions of the cystoblast and its stereotypic relationship with each other
and with the fusome (red). (A, right) The different regions of the
Drosophila germarium. The germline stem cells, and dividing
cystoblast comprise region 1. Region 2A contains post-mitotic 16 cell-cysts
(green). The boundary between regions 2A and 2B is marked by the follicle
cells migrating inwards to envelop the cyst and to form the egg chamber. The
red lines represent the spectrosome in the germline stem cells and the fusome
connecting all the cells in the dividing cystoblast and postmitotic cyst.
Region 3 of the germarium is characterized by the budding of newly formed egg
chambers. (B-G) Expression of Cyclin A (red) and the mitotic marker
phosphohistone 3 (green) in wild-type and in encore mutant germaria.
(B-D) In wild-type ovaries, Cyclin A is expressed in the stem cells and
dividing cystoblast in region 1 of the germarium. It rapidly declines in
post-mitotic cysts. (E-G) In encore mutant germaria, Cyclin A
expression persists after the end of mitosis. The arrow shows a cyst positive
for Cyclin A in a posterior area of the germarium.