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Fig. 1. Cyclin A protein expression in the germarium. (A, left) The four mitotic divisions of the cystoblast and its stereotypic relationship with each other and with the fusome (red). (A, right) The different regions of the Drosophila germarium. The germline stem cells, and dividing cystoblast comprise region 1. Region 2A contains post-mitotic 16 cell-cysts (green). The boundary between regions 2A and 2B is marked by the follicle cells migrating inwards to envelop the cyst and to form the egg chamber. The red lines represent the spectrosome in the germline stem cells and the fusome connecting all the cells in the dividing cystoblast and postmitotic cyst. Region 3 of the germarium is characterized by the budding of newly formed egg chambers. (B-G) Expression of Cyclin A (red) and the mitotic marker phosphohistone 3 (green) in wild-type and in encore mutant germaria. (B-D) In wild-type ovaries, Cyclin A is expressed in the stem cells and dividing cystoblast in region 1 of the germarium. It rapidly declines in post-mitotic cysts. (E-G) In encore mutant germaria, Cyclin A expression persists after the end of mitosis. The arrow shows a cyst positive for Cyclin A in a posterior area of the germarium.





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