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Fig. 2. Cyclin A promotes the encore extra division phenotype. (A-C) In
wild-type germaria Cyclin A (red) associates with the fusome (green stained
with phalloidin for actin) in dividing cystoblasts (arrow and inset). (D-F)
Association of Cyclin A with the fusome in encore mutant germaria
persists in posterior regions of the germarium (arrow and inset). (G)
encore mutant females raised at 25°C produce 55% of the egg
chambers with 32 cells. Reducing Cyclin A gene dose has no effect on
the encore cell division phenotype. However overexpression of the
HS-rux transgene in an encore mutant background reduces the
32 cells egg chamber phenotype from 55 to 25%. Additional reduction of
Cyclin A gene dose results in 20% of the egg chambers containing 32
cells. (H) Western blot showing that the amount of Cyclin A protein in
encore mutant extract is increased compared with the wild-type
extract. Equivalent amounts of germaria-enriched extract were loaded in both
lines as seen with the protein dye ponceau (pink).
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