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Fig. 2. Pnr is not essential for DV patterning of eye during embryogeneis.
Ush was misexpressed in eye by ey-GAL4; UAS-Ush
(ey>Ush) and cultures were shifted to 29°C during different stages
of development. (A) Three different restrictive temperature conditions used.
(B,D-F) Eye discs were stained for Wg or L (blue), Elav (red) and Ey (green).
(B-D) Cultures maintained at 29°C throughout development served as
controls and resulted in elimination of entire eye field (B) in eye disc and
(C) in adult eye. (D) Some weaker phenotypes of very small eye marked by
Elav-positive cells were also observed. (E) Maintenance at 18°C during
embryonic development and then shift to 29°C for subsequent development
also resulted in complete elimination of eye field. (F,G) When cultures were
shifted to 29°C during embryonic development to block pnr
activity and later allowed to develop at 18°C, they did not show any eye
suppression phenotype (F) in the eye disc and (G) in adult eye.
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