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Fig. 4. Loss of Ser gene function can abolish the eye fate. (A) Graphical
presentation of eye phenotypes generated by targeted misexpression of
ey-GAL4; UAS-SerDN (ey>SerDN) along
the time course of temperature shifts for the samples collected at every 12
hours of interval until the late third instar. Effect of dominant-negative
Ser (SerDN) was scored for its effect on eye fate
in the discs and phenotypes observed were classified into three main
categories: complete loss of eye fate (blue); loss of ventral eye pattern
(purple) and generation of two antennal fields (yellow). For each time window,
at least 20 discs were scored. (B,D,E,G) Eye discs were stained with Elav
(red) and with Ey (green) and Wg (blue). Misexpression of
ey>SerDN for 12-72 hours resulted in complete
elimination of eye disc (B), adult eye (C) and eye disc with a few
photoreceptors (D) (shown in blue in A). For 96-108 hours,
ey>SerDN resulted in preferential loss of ventral eye
in disc (E) and adult (F) (shown in purple in A). It has been suggested that
loss of Ser using the same ey>SerDN caused the
homeotic transformation of the antenna to the eye fate
(Kumar and Moses, 2001). (G)
ey>SerDN primarily showed suppression of the eye field
and also occasionally (3/35) results in the generation of two antenna fields
(shown in yellow in A). This may be due to `splitting' of the antenna field,
as evident from the mirror image duplication of Wg expression in the ventral
sector of antenna disc (AN).
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