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Fig. 4. Repression of Bmp4-signaling pathways requires high doses of Pitx2. (A-G) Whole-mount in situ analysis of 10.5 dpc Pitx2 ${delta}$ abc^null homozygous mutant (A), Pitx2 wild-type (B,E), ${delta}$ abc^null; ${delta}$ ab^hypoc (C,F) ${delta}$ abc^null; ${delta}$ ab (D,G) embryos with a Bmp4 probe. (H-K) Whole-mount in situ analysis of 10.5 dpc Pitx2 wild-type (H), ${delta}$ abc^null homozygous mutant (I), ${delta}$ abc^null; ${delta}$ ab^hypoc (J) and ${delta}$ abc^null; ${delta}$ ab (K) embryos with Msx2. (L-O) Whole-mount in situ of 10.5 dpc wild-type (L), Pitx2 ${delta}$ abc^null homozygous mutant (M), ${delta}$ abc^null; ${delta}$ ab^hypoc (N) and ${delta}$ abc^null; ${delta}$ ab (O) embryos with Msx1 showing normal (arrows) and expanded (arrowheads) areas of expression. ^*Areas in wild type with expanded expression in mutant. md, mandibular process; mx maxillary process.

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