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Fig. 4. Foetal and postnatal NLBs contain bipotential progenitors of enteric
neurons and glia. (A-C,G-I) Typical colonies generated from single GFP+ cells
isolated from foetal (A-C) and postnatal (G-I) NLBs on day 1 (A,G), day 5
(B,H) and day 10 (C,I). (D-F,J-L) Equivalent colonies immunostained with
TO-PRO-3 (blue; to reveal the cell nucleus), TuJ1 (red; to identify neurons)
and GFAP (green; to identify glia). The cell shown in D and J (day 1) had weak
GFP signal but lacked the characteristic TuJ1 and GFAP staining. To avoid
confusion with the GFP-specific signal, E and K (day 5) and F and L (day 10)
show sectors of colonies in which the retroviral transgene had been
extinguished. TuJ1+ cells were detected in day 5 and day 10 colonies but GFAP+
cells were detected only in day 10 colonies. Arrows in F and L point to two
neighbouring cells that express TuJ1 or GFAP. (M) Cells in foetal colonies
have higher proliferative capacity. Five-dayold colonies from foetal and
postnatal EPCs were stained with the mitotic marker H3p. The fraction of H3p+
cells in foetal colonies was higher relative to that of postnatal
colonies.
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