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Fig. 8. Effects of GDNF on the progeny of foetal and postnatal EPCs. (A) Day-5
clonal cultures of EPCs fixed and stained for H3p or TuJ1. In the upper part
of the graph, bars represent the percentage increase in the number of H3p+
cells in the presence of GDNF relative to those present in control (absence of
GDNF) culture conditions. In the lower part of the graph, bars represent the
percentage decrease in the number of TuJ1+ cells in the presence of GDNF
relative to those present in control cultures. (B-E) GDNF also promotes the
morphological differentiation of EPC progeny maintained in defined medium.
Foetal (B,C) and postnatal (D,E) EPCs were cultured for 4 days in complete
NCSC medium. Subsequently, they were cultured for a further 4 days in defined
medium (NCSC medium lacking CEE, FGF and EGF) in the absence (B,D) or presence
(C,E) of GDNF. GDNF promotes axonal growth and branching of neurons in both
foetal and postnatal EPC colonies.
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