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Fig. 2. An ectopic expression assay for Notch activity in the wing. Wing imaginal discs, with ventral bottom and anterior left, stained for expression of WG (magenta), GFP (green) and ß-galactosidase (red). Arrows point to ectopic dorsal expression; arrowheads point to absence of expression. Panels marked with a prime show a single channel of the double stain to the left. (A,B) GFP marks the ptc-expression stripe. Higher or lower Notch expression can affect the length of the ectopic Notch activation stripe (i.e. how far it extends towards the proximal wing), but not the width (Notch activation is always confined to the AP boundary). (A) ptc-Gal4 UAS-N.865 UASGFP. (B) ptc-Gal4 UAS-N.865 UAS-fng[22a]UAS-GFP. Notch activation in ventral cells is due to potentiation of Delta signaling by Fringe (Panin et al., 1997). (C-F) GFP marks wild-type cells; insets show a portion of the ptc expression stripe (red). (C) ptc-Gal4 UASN.865 UAS-lacZ with anterior Dl^rev10 clone. (D) ptc-Gal4 UAS-N.865 UAS-lacZ with posterior Dl^rev10 clone. (E) ptc-Gal4 UAS-N.865 UAS-lacZ with anterior Ser^rev2-11 clone. (F) ptc-Gal4 UAS-N[865]UAS-lacZ with posterior Ser^rev2-11 clone. This clone also disrupts normal Notch activation at the DV border.

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