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Fig. 4. Notch ligands are required for N EGF12f induced Notch activation. Wing discs stained for WG (magenta) or SER (red), and GFP or MYC (green). (A,C,E) Arrows point to ectopic expression inside clones; arrowheads point to its absence. (B,D,F,G) Arrows point to ectopic expression at the edge of clones. (A-G) MARCM clones, in which cells expressing Gal4 under tub-Gal4 control are identified by GFP expression. In C-G the clones are also mutant for Notch ligands. (A) tub-Gal4 UAS-GFP UAS-N-EGF12f[M4a]. (B) tub-Gal4 UAS-GFP UAS-N.865. (C) tub-Gal4 UAS-GFP UAS-N-EGF12f[M4a] Ser^rev2-11. (D) tub-Gal4 UAS-GFP UAS-N.865 Ser^rev2-11. (E) tub-Gal4 UAS-GFP UAS-N-EGF12f[M4a] Dl^rev10. (F) tub-Gal4 UAS-GFP UAS-N.865 Dl^rev10. (G) tub-Gal4 UAS-GFP Ser^rev2-11 Dl^rev10. (H) SER expression in Dl mutant clones (outlined by white dashes). Faint staining in some regions is from the peripodial epithelium. In a large clone that spans the DV boundary (1), SER expression is lost non-autonomously. In a narrow spanning clone (2), SER is not significantly affected. In a dorsal clone (3), SER expression is reduced but not absent. (I) SER expression is lost autonomously from Notch clones.

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