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Fig. 5. N-EGF12f is a functional receptor, but cannot rescue DV boundary formation. Wing discs stained for expression of WG (magenta), with MARCM clones expressing a Notch receptor under tub-Gal4 control and mutant for the endogenous Notch gene identified by GFP (green). (A) N55e11; tub-Gal4 UAS-GFP UASN.865. Notch activation is continuous through a clone that spans the DV border (arrow). (B) N55e11; tub-Gal4 UAS-GFP UAS-NEGF12f[M4a]. Notch is activated ectopically within a dorsal clone (arrow). (C) N55e11; tub-Gal4 UAS-GFP UAS-N-EGF12f[M4a]. Notch activation is disrupted at the DV boundary (arrowhead).





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