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Fig. 1. Localization of PGC precursor cells in early streak embryos. Explants of proximal epiblast at E6.5 (A) and E6.75 (D) cultured with recombinant human BMP4 for 72 and 66 hours, respectively. BMP4 stimulates PGC formation from E6.5 proximal epiblasts (unpublished results). ALP- and PGC7/stella-positive PGCs developed from E6.5 explants (B,C, arrowheads), but no such cells emerged from E6.75 explants (D). The same group of cells is shown in B and C. E6.75 extra-embryonic mesoderm generated ALP-positive PGCs (E). Scale bar: 160 µm (A,D,E) and 100 µm (B,C).





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