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Fig. 3. PGCs from Oct4-positive cell cluster in isolated extra-embryonic mesoderm at E6.75. (A-G) Time-lapse analysis of Oct4-GFP extra-embryonic mesoderm. (A-E,G) Images of cells expressing Oct4-GFP in extra-embryonic mesoderm on an STO feeder layer after (A) 0, (B) 12, (C) 15, (D) 30, (E) 36 and (G) 40 hours in culture. (F) ALP expression of explant in G. Arrowheads indicate ALP- and GFP-positive migrating PGCs. (H,I,J) Co-expression of PGC markers, (H) ALP, (I) PGC7 and (J) Oct4-GFP in PGCs (arrowheads) that developed from cultured E6.75 extra-embryonic mesoderm. Scale bar: 200 µm (A-G) and 100 µm (H-J).





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