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Fig. 5. E-cadherin expression in clustered PGC precursors at base of nascent
allantois. Lateral view of embryos at (A-D) mid-streak (E6.75), (E-H) mid- to
late streak (E7.0), and (I-L) early allantoic bud (E7.25) stage with anterior
to left. (A-D) Oct4-GFP (A) and E-cadherin (B) expression was slightly and
obviously lower in the distal portion of extra-embryonic mesoderm (dotted
line). Arrows indicate boundary of epiblast and extra-embryonic mesoderm;
exee, extra-embryonic ectoderm; exem, extra-embryonic mesoderm; epi, epiblast;
ve, visceral endoderm. (E-H) Cells expressing intense GFP (E, solid line) form
a cluster at the apical tip of the E-cadherin-expressing region (F) of
extra-embryonic mesoderm. (I-L) Committed PGCs expressing PGC7 (I) dispersed
in the most distal region in E-cadherin-expressing extra-embryonic mesoderm
(J). (C,G,K) TOTO-3 staining of nuclei and (D,H,L) merged images. Scale bar:
50 µm (A-D,I-L) and 25 µm (E-H).
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