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Fig. 4. Msx1 expression is specified by a threshold concentration of Bmp. One-cell
stage embryos were injected with a combination of 50 pg of Wnt5a mRNA and
different amounts of CM-Bmp4 mRNA, which are indicated in the figure. Animal
caps were dissected at stage 9 and the expression of msx-1, slug and
histone H4 was analyzed by RT-PCR when sibling embryos reached the neurula
stage 16. (A) Embryos and animal cap samples are shown. (B) Quantification of
the gel shown in A.
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