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Fig. 4. sma-9 is widely expressed. sma-9 upstream sequences (K)
were fused with a GFP reporter gene. (A-C) GFP expression in wild-type
transgenic animals. The transgenic animals display a strong fluorescence in
the VNC, pharynx and intestine, from stages L1 (not shown) to adult (A,B). L2
stage (C) animals show expression in the seam cells that disappears after L3
(Nomarski image in D). Arrows in C and D indicate seam cell nuclei. (E,G,I)
Immunostaining by anti-SMA-9 antibodies against the unique 70 aa C terminus
present in class II isoforms. (F,H,J) DAPI staining of the same animals. (E,F)
N2; (G,H) sma-9(wk55); (I,J) enlarged view of the anterior region of
a wild-type late L1 stage animal. In all animals, anterior is to the left,
arrowheads show pharyngeal nuclei, black arrows show intestinal nuclei and
white arrows show hypodermal nuclei. (K) Different sma-9 upstream
regions used to construct GFP reporters.
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