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Fig. 4. RA regulation of p21 in vivo and in vitro. Whole-mount staining with
antibodies against p21 revealed significantly reduced levels of p21 in
Raldh2-/- yolk sacs (B) compared with wild type (A).
Section staining revealed p21 expression predominantly in endothelial cells in
wild-type yolk sacs (C, arrows); p21 was essentially absent in
Raldh2-/- yolk sac (D). Endothelial cells were cultured
for up to 72 hours in the presence or absence of 1 µM RA. Western analyses
revealed that RA induced the expression of p21 in endothelial cells within 8
hours (E).
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