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Fig. 1. echinoid mutants display additional es organs and SOPs. (A,B)
Scanning electron microscopy of the adult thorax. (A) Wild type. (B)
edlH23/edl(2)k01102
showing additional macrochaetae around each wild-type macrochaete location.
(C) Light micrograph of sca-Gal4; UAS-ed thorax. Arrows
indicate the positions where macrochaetae should be present. An arrowhead
indicates two macrochaetae that have arisen from the same PNC in place of one.
(D-J) Confocal microscopy for immunohistochemical detection of (D-G) Sens, and
(H-J) Ac (green) and Sens (red) in third larval instar wing discs. (D) Wild
type. (E)
edlH23/edl(2)k01102.
(F,G) Higher magnification views of scutellar region in (D,E). Note that two
SOPs are present at each position (one of the SOPs in F has divided and
appears as two adjacent cells). (H) Wild type. (I)
edlH23/edl(2)k01102
showing no difference in Ac level or pattern compared with wild type. (J)
sca-Gal4; UAS-ed wing disc at puparium formation. Arrows
indicate proneural clusters that have persisted much later than normal in the
absence of SOP specification.