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Fig. 1. Control experiments. (A) Morphology of an uninjected control larva. Trunk and tail are indicated by a bracket and a broken line, respectively. (B) Morphology of a larva developed from an egg injected with Ci-Bra morpholino. (C) Expression of notochord marker Ci-talin (arrowhead) in a tailbud embryo visualized by whole-mount in situ hybridization. (D) The absence of Ci-talin expression (white arrowhead) in a tailbud embryo injected with Ci-Bra morpholino. (E) Recovery of Ci-talin expression (arrowhead) in an embryo at the tailbud stage co-injected with Ci-Bra morpholino and its mRNA. Scale bars: 100 µm for A,B; 50 µm for C-E.





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