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Fig. 3. Ectopic Wg signalling transforms the identity and cell morphology of the PE
into wing hinge. (A) wg-expressing clones (green, 48-72 hours AEL)
transform both cell autonomously and non-autonomously squamous peripodial
cells into columnar cells, as seen both in a surface view and a longitudinal
section of the outlined clone. Arm (red) and TO-PRO-3 (blue) staining reveal
cell shape and tissue structure (apical membranes of both sides of the disc
separated by a dotted line in the section s1). The arrowheads in the section
indicate clones in the wing-notum side that do not transform the shape of
apposed squamous cells. (B) The transformations extend 7-15 cell diameters
away from the clone (green, 48-72 hours AEL). The severity of the
transformation decreases the further the affected cell is from the clone (see
inset). (C) These clones (48-72 hours AEL) eliminate Ubx expression
(red) in peripodial transformed cells (outlined clone and inset) and cubic
cells (arrowhead). (D) By contrast, in Arm
overexpression clones
(24-48 hours AEL) both the transformation in shape and the elimination of
Ubx expression are only cell autonomous (inset). wg
expressing clones (green) induce expression of hinge markers in the peripodial
side of the wing disc, such as ds (E), zfh-2 (F) and
nub (G). (E) In these clones ds (red) is induced in cells
both inside and outside the clone (see longitudinal section s2). Clone
generated 24-48 hours AEL. (F) Clones in squamous (outlined clone) and
proximal cubic cells (arrowhead) induce cell autonomous (see transversal
section s3) and non-autonomous expression of zfh-2 (red). Clone
generated 24-48 hours AEL. (G) Ectopic Wg (48-72 hours AEL), however, induces
only autonomous expression of nub (red), which is expressed in the
wild-type wing blade and proximal hinge. Notice that the expression of Nub
induced is mostly cytoplasmic.
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