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Fig. 4. Ectopic co-expression of wg and vg transforms the PE into
wing blade. (A) wg-vg expressing clones (green, 24-48 hours AEL)
transform squamous cells into columnar wing blade cells (see longitudinal
section s1 through the outlined clone). Vg (blue) is present only in cells of
the clone, while nub (red) is expressed both cell autonomously and
non-autonomously. (B) wg-vg expressing clones generated 24-48 hours
AEL induce the expression of the hinge marker zfh-2 in an exclusively
non-autonomous way (see transverse section s2). The arrowheads indicate a
clone in the wing-notum side that neither induces zfh-2 expression in
apposed peripodial cells nor affects their morphology. Note that the clone in
the peripodial side does not induce zfh-2 expression in the
wing-notum side either. Arrows indicate untransformed peripodial nuclei. (C)
Clones generated 48-72 hours AEL promote autonomous expression of the wing
pouch genetic marker dll. In contrast to earlier clones, the induced
expression of nub is exclusively non-autonomous (insets at higher
magnification).
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