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Fig. 3. HLH-14::GFP is expressed in the PVQ/HSN/PHB neuroblasts and their descendants. All images in this series show a ventral view. Anterior is at the upper left corner and posterior is at the lower right corner. Images in this series reveal posterior embryonic HLH-14::GFP expression. Nomarski photomicrographs (A,C,E,G,I,K) and the corresponding fluorescence photomicrographs (B,D,F,H,J,L) of wild-type embryos containing a gmIs20[hlh-14::gfp] transgene. (A,B) HLH-14::GFP is expressed in the right PVQ/HSN/PHB neuroblast, ABprapppa (arrow). (C,D) GFP expression of the same embryo shown in A and B, showing a different focal plane. HLH-14::GFP is expressed in the left PVQ/HSN/PHB neuroblast, ABplapppa (arrow). (E,F) HLH-14::GFP is expressed in the daughter cells of the PVQ/HSN/PHB neuroblasts: the anterior PVQ neuroblasts (open arrows) and the posterior HSN/PHB neuroblasts (closed arrows). We have tentatively identified the central GFP-expressing cell as the neuroblast C.aapa (arrowhead). (G,H) HLH-14::GFP is expressed in the anterior daughter of the right HSN/PHB neuroblast, a cell fated to die (closed arrow), and in its larger sister cell, the right HSN/PHB precursor (open arrow). The asterisk indicates the right PVQ neuroblast, which is out of focus. (I,J) HLH-14::GFP is expressed in the daughters of the left HSN/PHB neuroblast (arrows analogous to H). (K,L) HLH-14::GFP is expressed in the daughters of the PVQ neuroblast, the anterior PVQ neurons (closed arrows) and their posterior sisters, which are fated to die (open arrows). Scale bar: 5 µm. (M) Cell lineage depicting the PVQ/HSN/PHB neuroblast and its descendants.

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