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Fig. 1. Map of dpy-21 molecular lesions and levels of dpy-21
transcripts throughout development. (A) The intron-exon gene structure of
dpy-21. The molecular changes for seven dpy-21 mutant
alleles are indicated. Genetically, dpy-21(e428) is the most severe
mutation, causing 17% larval lethality and dumpy, egg-laying defective adult
survivors, some with a protruding vulva. (B) Northern blot of mRNA isolated
from wild-type embryos, L1, L2, L3 and L4 larvae, and young adults without
embryos. The blot was hybridized with a probe to the first 3347 nucleotides of
the dpy-21 transcript and a myo-1 probe to measure
pharyngeal myosin transcript levels as a loading control. The dpy-21
transcript is expressed throughout development, with the highest transcript
levels occurring during embryogenesis.
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