|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. DPY-21 is not recruited to her-1 regulatory regions in XX animals,
unlike other components of the dosage compensation complex. (A,B) False-color
confocal immunofluorescence images of wild-type embryos (A) or gut cell nuclei
(B) carrying her-1 extrachromosomal arrays that contain multiple
copies of her-1 regulatory regions, lac operator repeats
(lacO) and a transgene encoding a LacI-GFP fusion protein. LacI-GFP
repressor binding to lacO permits array detection by GFP antibodies.
For each embryo or gut cell nucleus, a single z-section is shown.
Embryos and gut cell nuclei were stained with DAPI (gray) and antibodies to
DPY-21 (green), SDC-3 (red) and GFP (blue). The fourth column shows the
superimposition of the DPY-21 and SDC-3 images. (A) In embryos that have
activated dosage compensation, both DPY-21 and SDC-3 co-localize with the X
chromosome, which is denoted by an asterisk in the inset. By contrast, SDC-3,
but not DPY-21, localizes to her-1 regulatory regions on the arrays.
(B) In adult gut cell nuclei, DPY-21 co-localizes with SDC-3 on X chromosomes,
but does not co-localize with SDC-3 on her-1 regulatory regions.
Thus, DPY-21 participates directly in the chromosome-wide repression of X but
not in the gene-specific repression of her-1. Scale bars: 5
µm.
|
