DEV00904 Supplemental Figures

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Wg signaling influences growth during wing development. (A,D) Confocal sections of third instar wing imaginal discs labeled with antibodies to visualize (A,B) Cut (green) and Myc epitope tag (red), or (C,D) Notum antibody (red). (A) Control, apterous Gal4. (B) Reduction of Wg signaling in the D compartment of an ap-Gal4 UAS-nkd-myc (II) wing disc. Note that Notch activation is maintained at the V boundary visualize by the expression of the target gene Cut (green). (C) Control wing expressing a mutant form of Notum without enzymatic activity (Giraldez et al., 2002). (D) Reduction of Wg signaling in the D compartment of an ap-Gal4 UAS-NotumGT wing disc. Note the small D compartment, yet the Notch pathway at the V boundary remains active to induce Cut expression (green). (E,F) Wild-type and mutant adult wing corresponding to the genotypes in C and D, respectively. Note the small dorsal wing that results as consequence of the reduction of Wg signaling.

• Supplemental Figure 2 -

  Fig. S2. Activation of the Notch pathway induces Wingless expression and tissue growth in a specific time window during development. Positively marked clones expressing CD8-GFP (green) and N^Intra, at different time points during larval development 36±4 hours AEL (A), 48±4 hours AEL (B), 60±4 hours AEL (C) and 72±4 hours AEL (D). Wingless expression is shown in red (left) or in black (right). Clones generated before 48±4 hours AEL induced tissue overgrowth at a high frequency (60% of the clones in the distal pouch or hinge regions) concomitantly with wingless induction (A,B). Clones expressing N^Intra later than 60±4 hours AEL presented a reduce ability to induce tissue overgrowth and to activate wingless expression away from the DV compartment boundary (C,D). Scale bars: 50 mm.