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Fig. 2. The targeted mutation of the RARE abrogates RA-response in tissue culture.
F9 embryocarcinoma cells were transfected with 1.5 µg of wild-type (Wt)
Cdx1 reporter, a reporter with a mutated RARE (Mut) or an RARE
equivalent to the targeted mutation after Cre-mediated recombination (Lox
mut). Twenty-four hours after transfection, cells were treated with vehicle or
10-6 M RA and luciferase activity assessed 24 hours post-treatment.
Results, from independent triplicate experiments, were expressed as fold
RA-induction relative to vehicle treated cells. Each transfection was repeated
at least twice with similar results.
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