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Fig. 5. RA induction of Cdx1 in RARE-null mutants is independent of de
novo protein synthesis. RARE-null mutant E8.5 embryos were cultured for 4
hours with vehicle (A), 10-6 M RA (B), cycloheximide (C) or
cycloheximide plus RA (D; all cycloheximide cultures were initiated 30 minutes
prior to addition of RA). Note that cycloheximide did not prevent RA induction
of Cdx1 in the RARE-null mutants (compare D with B). The embryos are
arranged to represent stage-matched samples between the four panels, with
those on top possessing 8-12 somites and those on the bottom having between
one and eight somites.
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