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Fig. 1. vcs mutants show temperature-dependent defects in leaf
development. (A-C) Plants grown at 22°C; 15-day wild type (A) has broad
leaves, whereas the 15-day vcs mutant (B) produces narrow curled
leaves. Following prolonged growth (C; 25-day shown), vcs develops
flowers, but no seeds are produced. (D-F) Plants grown at 16°C; 16-day
wild type (D) produces broad leaves, and the 16-day vcs produces
broad, but pointed leaves (E). At this temperature, vcs can be grown
on soil, produce flowers and set seed (F), but the mutant plant has short
inflorescence stems and reduced apical dominance (wild type on the left and
vcs on the right). (G-I) Plants grown at 29°C; 11-day wild type
produces broad leaves (G), whereas the 11-day vcs mutant appears
chlorotic and produces small epinastic leaves (H). Prolonged growth at
29°C in vcs results in a few additional leaf-like organs, and an
enlarged callus-like apex (I). (J-P) Roots of wild type and vcs
mutants: (J,K) 16°C-grown 7-day wild type and vcs, respectively;
(L,M) 22°C-grown 4-day wild type and vcs, respectively; (N-P)
29°C-grown 4-day wild type (N) and vcs (O,P). A high
magnification image of the vcs root (P) shows the swollen root hairs.
Scale bars: (A-O) 1 mm; (P) 100 µm.
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