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Fig. 10. Molecular identification of VARICOSE. (A) Mapping strategy within
a 1.6 MB region of chromosome 3. The approximate positions of the
polymorphisms used in this study for high resolution mapping of recombination
breakpoints are indicated at the top. Using 750 vcs F2 mapping cross
plants, we identified six recombinants at the polymorphism indicated as
VCSA, and 48 at the VCSI polymorphism. The DNA from the
plants containing recombinant chromosomes were further analyzed at
polymorphisms VCSB, MDC11, MRP15, and MAG2. The number of
chromosomes that were still heterozygous at each position is indicated. (B)
Depiction of the VCS gene; black boxes represent exons, the arrow on
the bar indicates transcription direction for orientation of the 5' end
of this gene. The position and nature of mutations in each vcs allele
is indicated.
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