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Fig. 6. Shows the effect of an anti-Kit antibody or an isotype matched control
antibody (J1.2) on 48 hour survival of either E10.5 (A) or E12.5 (B) germ
cells. Survival is shown first as % of the number of PGCs counted in each
embryo sample from a single litter at T0, and second, as means
(±s.e.m.) for all embryos of the same genotype in the litter. In the
E10.5 sample (A), 15 µg/ml of control (blue bars) and anti-Kit (red bars)
were used. In the E12.5 sample (B), two concentrations of antibody were used;
15 µg/ml and 50 µg/ml. The effects of each dose are shown separately on
the charts. Antibody concentrations of 15 µg/ml are represented by blue
(control antibody) and red (anti-Kit antibody) bars (as in A), while the
effects of 50 µg/ml of control or anti-Kit are shown as yellow and green
bars, respectively. The anti-Kit antibody causes a significant reduction in
germ cell survival at 48 hours in both E10.5 and E12.5
Bax+/+ and Bax+/- embryos, but not in
Bax-/- embryos. In the E12.5 litter, there was only one
Bax+/+ embryo, so there is no s.e.m.
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