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Fig. 4. FGF2-mediated oligodendrocyte induction is dependent on MAP kinase
signalling and is inhibited by BMP4. (A) Isolated E14 rat dorsal spinal cord
cultures were examined for expression of oligodendrocytes (O4), astrocytes
(GFAP), neurones (ß-tubulin) and smooth muscle actin (SMA) at 4-days
post-plating, following exposure to FGF2 and BMP4 (0.1-10 ng/ml), MEK
inhibitor (UO126) or PI3K pathway inhibitor (LY294002). BMP-treated cultures
show a dose-dependent reduction of oligodendrocytes and astrocytes, and an
induction of SMA. UO126-treated cultures show a similar reduction in
oligodendrocytes (*=P<0.01;
**=P<0.05). (B) Immunomicrograph showing
oligodendrocyte (O4) and astrocyte (GFAP) generation following 96 hours of (I)
FGF2 and (II) FGF2 + BMP4 (10 ng/ml) treatment of E14 dorsal cultures.
BMP4-treated cultures have no oligodendrocytes and reduced numbers of
astrocytes following differentiation in control media after withdrawal of
4-day FGF2 and BMP4 treatment; compare with cultures treated with FGF2 alone.
Immunomicrographs showing (III) neuronal (ß-tubulin) generation following
FGF2 stimulation of E14 dorsal cultures, and (IV) smooth muscle actin-positive
staining cells derived from FGF2 and BMP4 (10 ng/ml) treated cultures.
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