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Fig. 5. zpg is required for Dpp-induced over-proliferation of early germ
cells. Flies of the indicated genotypes were either dissected (C,E), or
subjected to heat shock prior to dissection (A,B,D,F), and then stained with
anti-Vasa (green) and mAb 1B1 antibody (red; A,B) or anti-pMad (red; C-F).
After heat shock, hs-dpp; zpg heterozygous ovaries were
filled with many single germ cells (A). hs-dpp, zpg homozygous mutant
ovaries did not show any proliferation of single germ cells (B). Staining of
wild-type (OR) germaria revealed p-Mad staining in early germ cells (C),
whereas all germ cells have nuclear p-Mad when Dpp is overexpressed (D).
zpg cells also have nuclear p-Mad, whether Dpp is overexpressed (F)
or not (E). Scale bar: in A, 20 µm for A,C,D; in B, 50 µm.
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