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Fig. 6. An average of 3.9 single cells do not stain with BamC antibody. (A)
Individual germaria were scored for the number of cells that contain a
spherical spectrosome and do not stain with BamC antibodies. The graph
illustrates the number of germaria (y axis) that contained between 1
and 6 of such cells (x axis). (B) Wild-type ovaries were stained with
anti-Vasa (blue), with the mAb 1B1 antibody (green) and with anti-BamC (red).
Four cells that possess a single spectrosome but do not stain for BamC can be
observed at the anterior of the ovariole (arrowheads). A cyst, which does
stain with BamC antibody, can be seen just posteriorly (arrow). Scale bar: 20
µm. (C) An illustration of the possible outcomes of mitotic recombination
in a pre-cystoblast or a GSC (see arrows). Cells carrying 0 copies of the
lacZ transgene are depicted in white, whereas those carrying 1 or 2
copies of the transgene are light or dark green, respectively. (D) A germarium
representing a GSC mitotic recombination event. Arrowheads mark the GSC and
its progeny lacking lacZ (only one daughter cell is in plane). An
arrow marks the `twin spot', which strongly expresses lacZ.
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