Fig. 3. Mbt mutations disrupt adherens junction integrity during pupal photoreceptor cell morphogenesis. Shown are proximal-to-distal projection views of wild-type (WT) eye imaginal discs at 37% p.d. (left column) and 50% p.d. (middle column) stained with anti-Mbt and anti-Arm antibodies. Staining of both overlap in the developing photoreceptor cells and also in the surrounding pigment and bristle cells (WT merge). Three-dimensional reconstructions of single ommatidial clusters at 37% p.d. (left column, insets) and 50% p.d. (right column) illustrate the massive distal (top)-to-proximal (bottom) extension of the AJs during pupal development. Anti-Arm staining of mbt^P1 pupal eye discs (mbt^P1, -Arm) shows the disorganisation of the AJs. In the proximal-to-distal projection view, lateral extensions are evident (arrow) and the AJs fail to extend (inset). At 50% p.d., Armadillo accumulates in patches (right picture). Scale bars: 10 µm.

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