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Fig. 4. Pax1 induces Bapx1 expression and chondrogenesis in
explants of chick PSM. Explants of PSM isolated from stage HH10 chick embryos
were infected with the control RCAS-A-AP retrovirus (AP; A, lanes 1,3; B, lane
1) or with the RCAS-A-Pax1 retrovirus overexpressing mouse Pax1 (A, lanes 2,4;
B, lane 2), and cultured in the absence (A, lanes 1,2; B) or the presence (A,
lanes 3,4) of 500 ng/ml Shh. After 5 days of culture, explants were harvested
and analyzed by RT-PCR. The PCR products for chick Bapx1, aggrecan,
Gapdh and mouse Pax1 are shown.
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