Fig. 1. Gene targeting at the Ldb1 locus. (A), Partial restriction map of the wild-type Ldb1 locus, the targeting vector, and disrupted Ldb1 allele. A gene conferring neomycin-resistance replaces the deleted Ldb1 coding sequences (exons 3-9) following homologous recombination. (B) Southern blot analysis of targeted ES cells. Two independent clones TS17 (lanes 2, 5) and TS153 (lanes 3, 6) were used for blastocyst injection. TS12 is a wild-type control (lanes 1, 4). EcoRV- and HindIII-digested ES cell DNA was used for Southern analyses with 5' and 3' probes respectively. The 5' probe hybridized to 4.7 kb and 6.2 kb restriction fragments generated from the wild-type and the mutated alleles respectively, while the 3' probe detected a 6.1 kb wild-type and a 6.6 kb mutant fragment.

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