Fig. 4. Expression of putative Shh target genes in ozd limb buds. Both Ptc1 (A,B) and Gli1 (C,D) expression is undetectable in ozd limb buds at early (A,C) or later stages (B,D). (E-F) Gli3 expression is normal in mutant limbs at st. 18/19 (E), but abnormally extends to the posterior border at later stages (F). (G,H) dHAND expression is normal in early stage ozd wing buds (G), but is posteriorly restricted at later stages. (I,J) ozd limb buds express Bmp2, but expression levels are reduced and the spatial domain posteriorly restricted relative to wild type. In every panel anterior is up.

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