Fig. 5. Expression of Hoxd11, Hoxd12 and Hoxd13 in ozd limb buds. (A-D) Hoxd11 expression in wild-type and ozd limbs. (A,B) Up to st. 25, Hoxd11 shows a normal pattern of expression in the mutant wing, although its expression level is slightly reduced. (C,D) From st. 27, Hoxd11 expression in the ozd wing is confined to the posterior border. (A-D) In ozd leg buds, Hoxd11 expression is more affected, showing a reduced domain of expression by st. 21/22 (A), and becomes undetectable by st. 24/25 (B-D). (E-H) Hoxd12 expression in wild-type and ozd limbs. (E) The pattern of Hoxd12 expression is close to normal in st. 21/22 ozd wing buds, but expression levels are reduced. (F-H) From st. 24/25, Hoxd12 becomes confined to the posterior border of the mutant wing. (E-H) Hoxd12 expression in the ozd leg bud is only observed at early stages and at very reduced levels. (I-L) Hoxd13 expression in wild-type and ozd limbs. (I) Hoxd13 expression occurs at low levels and is posteriorly restricted in st. 21/22 ozd wing and leg buds, relative to wild type. (J-L) From st. 23/24 Hoxd13 expression becomes undetectable both in wing and leg buds. (K-L) Expression is re-initiated at st. 27 in the distal mesoderm of ozd legs. In all the panels anterior is up.

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