Fig. 3. Time course of Wnt2b overexpression and induction of LEF1 expression by in ovo electroporation. (A-D) In situ hybridization of normal E3 retina probed with chicken Wnt2b (A), Fzd4 (B), Fzd5 (C) and LEF1 (D). (E-J) In situ hybridization of the electroporated embryos probed with the overexpressed mouse Wnt2b (E-G) and LEF1 (H-J). The embryos were fixed 12 hours (E, H), 24 hours (F, I) and 48 hours (G,J) after the electroporation. The upregulation of LEF1 mRNA was detected 12 hours after the initiation of exogenous mouse Wnt2b expression, which continued for at least 24 hours. di, diencephalon; eb, eye bud. Scale bars: 100 µm.

Return to article