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Fig. 3. Interaction of the PDF2 gene product with the L1 box in vitro. Gel
retardation assays were performed using a labeled 21-bp L1 box probe (L1)
derived from the PDF1 promoter, together with the MBP alone or the
PDF2 fusion protein (MBP-PDF2) as indicated. The wedge indicates increasing
amounts of 100-, 300- and 1000-fold molar excesses of unlabeled L1 probe DNA
in competition assays. An assay using a mutated probe (mL1) with MBP-PDF2 is
also shown.
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