Fig. 4. Regulation by wg. In wild-type discs, CG17278 (A), Cyp310a1 (D) and CG8381 (G) are expressed broadly in the wing pouch but excluded from cells at the DV boundary. Misexpression of wg in the dorsal and ventral wing pouch (71B-GAL4; UAS-wg) inhibits expression of the genes in a broad band of cells in the wing pouch (B,E,H). Inhibition of Wg signaling at the DV margin (C96-GAL4; UAS-DNdTcf) induces more cells at the margin to express the genes (C,F,I). At the center of a wild-type disc approximately four rows of cells do not express CG17278 (A, arrowheads), whereas expressing cells are adjacent at this point when Wg is inhibited (C, arrowheads). When Wg is inhibited, Cyp310a1, expression spans the DV boundary for its entire length (F). Expression of CG8381 in the wild-type wing pouch is excluded from a band of six to seven cells at the DV boundary (G, arrowheads) and this is reduced to approximately two cells at the equivalent position when Wg is inhibited (I, arrowheads).

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