Fig. 9. SPW1 expression in wild-type, dl-sup1 and spw1-1 flowers. (A-D, I-L) Wild-type, (E-H) dl-sup1 and (M-P) spw1-1 flowers. (A-H) Non-radioactive, and (I-P) radioactive in situ hybridization. (A) SPW1 RNA was detected in the floral meristem where incipient lodicule (arrowhead) and stamen primordia were formed. (B-D) SPW1 expression was detected in developing stamen and lodicule primordia but not in the carpel primordium (B,C) and (D) in the anthers (arrows) and filaments but not in the young ovary (ov) with developing stigma. (E) The expression pattern of SPW1 in dl-sup1 background was indistinguishable from the wild type at the stage when whorl 4 showed no sign of differentiation. (F,G,H) Ectopic expression of SPW1 was detected in the region where ectopic stamens are formed (arrowheads). Ectopic expression of SPW1 is not detected in the center of the floral meristem throughout flower development (arrows). (I,K,M,O) Bright-field, and (J,L,N,P) dark-field micrographs. (I,J) Floral meristem of a developing wild-type flower. (K,L) A young wild-type flower with developing floral organ primordia. (M,N) Floral meristem of a developing spw1-1 flower. (O,P) A young spw1-1 flower with developing floral organ primordia. le: lemma, pa: palea, lo: lodicule, st: stamen, ca: carpel, ov: ovule. The numbers 2, 3 and 4, indicate the position of whorls 2, 3 and 4, respectively. Bars. 30 µm.

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