Fig. 7. CNS and tracheal defects in tdf and mbf1 mutants and genetic interaction between the two genes. CNS and tracheal lumen networks were stained in stage 16 embryos of the indicated genotypes with the mAb BP102 (left) and the mAb 2A12 (right), respectively. Arrowheads represent the defects in the CNS and tracheal network formation. The penetrance of each phenotype (%) is shown in the parentheses. P[MBF1⁺] is a rescue construct placed on the mbf1 chromosome, providing a wild-type copy of the gene. The tracheal defects in tdf^P3 was variable from embryo to embryo. (D) A mild phenotype seen in some embryos. We also observed more severe phenotype in other embryos (data not shown, but see Fig. 1E by Eulenerg and Schuh (Eulenerg and Schuh, 1997). This could be due to variable and partial rescue of the lack of zygotic tdf function by maternally supplied tdf, because the lack of maternal tdf enhances the zygotic tdf phenotype (Eulenberg and Schuh, 1997). Total numbers of examined embryos were: A, 138; B, 136; C, 98; D, 112; E, 89; F, 108; G, 156; H, 98; I, 120; J, 132; K, 118; L, 128; M, 116; N, 121.

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